|Prof. Yves-Alain Barde
Biozentrum, University of Basel
CH-4056 Basel / Switzerland
Yves Barde is Professor of Neurobiology and worked at the Biozentrum of the University of Basel until 2013. He is a developmental neurobiologist working on growth factors for neurons. The lack of suitable in vitro assay systems to study neurons of the central nervous system (CNS) led his group to use mouse of embryonic stem (ES) cells to generate neurons. Unlike previously thought, it turned out that ES cells can be converted in vitro into defined and homogenous neuronal progenitors that go on to differentiate into essentially one population of CNS neurons. This system provides a novel opportunity to dissect mechanisms relevant to the CNS, both during normal development and in disease. For example, it allowed the demonstration that the lack of a single transcription factor in ES cells is sufficient to entirely shift the type of neurons generated by ES cell-derived progenitors. It also led to the discovery of a new mechanism causing axonal degeneration. Recently, the group started using human embryonic stem cells to test whether uniform populations of human neurons can be generated with the techniques developed with mouse ES cells. This would open the possibility to study human neurons in vitro, both during normal development and in diseases.
Yves Barde co-founded the Basel Stem Cell Network, a Competence Centre of the University of Basel.
Since September 2013, Yves Barde is Professor at the Cardiff School of Biosciences of the Cardiff University.
Original publications related to stem cells
- Bibel M, Richter J, Schrenk K, Tucker KL, Staiger V, Korte M, Götz M. and Barde YA. (2004). Differentiation of mouse embryonic stem cells into a defined neuronal lineage. Nature Neuroscience 7:1003-1009.
- Bibel M, Richter J, Lacroix E. and Barde YA. (2007). Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells. Nature Protocols 2:1034-1043.
- Plachta N, Annaheim C, Bissière S, Lin S, Rüegg M, Hoving S, Müller D, Poirier F, Bibel M. and Barde YA. (2007). Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nature Neuroscience 10:712-719.
- Nikoletopoulou V, Plachta N, Allen ND, Pinto L, Götz M. and Barde YA. (2007). Neurotrophin receptor-mediated death of misspecified neurons generated from embryonic stem cells lacking Pax6. Cell Stem Cell 1:529-540.
- Rauskolb S, Zagrebelsky M, Dreznjak A, Deogracias R, Matsumoto T, Wiese S, Erne B, Sendtner M, Schaeren-Wiemers N, Korte M. and Barde YA. (2010). Global deprivation of brain-derived neurotrophic factor in the CNS reveals an area-specific requirement for dendritic growth. J Neuroscience 30:1739-49.
- Nikoletopoulou V, Lickert H, Frade JM, Rencurel C, Giallonardo P, Zhang L, Bibel M. and Barde YA. (2010). Neurotrophin receptors TrkA and TrkC cause neuronal death whereas TrkB does not. Nature 467:59-63.
- Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu MS, Deogracias R, Gundelfinger ED, Kojima M, Nestel S, Frotscher M. and Barde YA. (2012). BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. J Cell Biol. 196:775-88.
- Deogracias R, Yazdani M, Dekkers MP, Guy J, Ionescu MC, Vogt KE, Barde YA. (2012). Fingolimod, a sphingosine-1 phosphate receptor modulator, increases BDNF levels and improves symptoms of a mouse model of Rett syndrome. Proc Natl Acad Sci USA 109:14230-5.
Reviews related to stem cells
- Götz M. and Barde YA. (2005). Radial Glial Cells Defined and Major Intermediates between Embryonic Stem Cells and CNS Neurons. Neuron 46:369-372.
- Affolter M. and Barde YA. (2007). Self-renewal in the fly kidney. Developmental Cell 13:321-322.
- Dekkers MP, Barde YA. (2013). Developmental biology. Programmed cell death in neuronal development. Science 340:39-41.